MCAM antibody

Catalog No. ABIN614548

This Mouse Monoclonal antibody specifically detects MCAM in FACS, WB, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MCAM antibody (ABIN614548)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: P1H12

Product Details anti-MCAM Antibody

Specificity: Recognizes Human CD146 also known as MUC18. The antibody P1H12 has been reported to cross-react with Mouse, Rabbit, Canine, but not Rat.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Immunization of mice with HUVEC

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing ≤ 0.09 % Sodium Azide, BSA and/or Gelatin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: Human CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50 % of the mass from carbohydrates.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Gene ID: 4162

NCBI Accession: NP_006491

UniProt: P43121